Publications

2023

Huhtinen, O. A., Salbo, R., Lamminmäki, U. & Prince, S. Selection of Biophysically Favorable Antibody Variants Using Modified Flp-In CHO Mammalian Display Platform. Frontiers in Bioengineering and Biotechnology 11, 1170081 (2023).
de Lucas, Á. G., Lamminmäki, U. & López-Picón, F. R. ImmunoPET Directed to the Brain: A New Tool for Preclinical and Clinical Neuroscience. Biomolecules 13, 164 (2023).

2022

Negi, P. et al. Time-resolved fluorescence based direct two-site apoA-I immunoassays and their clinical application in patients with suspected obstructive coronary artery disease. Frontiers in Cardiovascular Medicine 9, 912578 (2022).
Kulmala, A., Lappalainen, M., Lamminmäki, U. & Huovinen T. Synonymous Codons and Hydrophobicity Optimization of Post-translational Signal Peptide PelB Increase Phage Display Efficiency of DARPins ACS Synthetic Biology 11, 3174–3181 (2022).
Islam, M. K. et al. Integrins are enriched on aberrantly fucosylated tumour‐derived urinary extracellular vesicles. Journal of Extracellular Biology 1, e64 (2022).
Mehdi, F. et al. Generation of a Large Repertoire of Monoclonal Antibodies against Dengue Virus NS1 Antigen and the Development of a Novel NS1 Detection Enzyme-Linked Immunosorbent Assay. The Journal of Immunology 209, 2054–2067 (2022).
Pakharukova, N. et al. Archaic chaperone–usher pili self-secrete into superelastic zigzag springs. Nature 609, 335–340 (2022).
Peltomaa, R., Abbas, A., Yli-Mattila, T. & Lamminmäki U. Single-step noncompetitive immunocomplex immunoassay for rapid aflatoxin detection. Food Chemistry 392, 133287 (2022).
Hietanen, E. et al. Isolation and characterization of phage display-derived scFv antibodies against human parechovirus 1 VP0 protein. Scientific Reports 12, 13453 (2022).
Peltomaa, R., Barderas, R., Benito-Peña E. & Moreno-Bondi, M.C. Recombinant antibodies and their use for food immunoanalysis. Analytical and Bioanalytical Chemistry 414, 193–217 (2022).

2021

Leivo, J., Vehniäinen, M. & Lamminmäki, U. Phage Display Selection of an Anti-Idiotype-Antibody with Broad-Specificity to Deoxynivalenol Mycotoxins. Toxins 13, 18 (2021).
Brockmann, E-C. et al. Combinatorial mutagenesis with alternative CDR-L1 and -H2 loop lengths contributes to affinity maturation of antibodies. New Biotechnology 60, 173-182 (2021).
Akter, S. & Lamminmäki, U. A 15-min non-competitive homogeneous assay for microcystin and nodularin based on time-resolved Förster resonance energy transfer (TR-FRET). Analytical and Bioanalytical Chemistry 413, 6159–6170 (2021).
Islam, M. K. et al. Detection of bladder cancer with aberrantly fucosylated ITGA3. Analytical Biochemistry 628, 114283 (2021).
Mehdi, F. et al. Development of a fast SARS-CoV-2 IgG ELISA, based on receptor-binding domain, and its comparative evaluation using temporally segregated samples from RT-PCR positive individuals. Frontiers in Microbiology 11, 618097 (2021).
Strand, J. et al. Humanization, Radiolabeling and Biodistribution Studies of an IgG₁-Type Antibody Targeting Uncomplexed PSA for Theranostic Applications. Pharmaceuticals 14, 1251 (2021).
Veach, D. R. et al. PSA-Targeted Alpha-, Beta-, and Positron-Emitting Immunotheranostics in Murine Prostate Cancer Models and Nonhuman Primates. Clinical Cancer Research 27, 2050-2060 (2021).
Negi, P. et al. Three two-site apoA-I immunoassays using phage expressed detector antibodies – Preliminary clinical evaluation with cardiac patients. Journal of Pharmaceutical and Biomedical Analysis 194, 113772 (2021).
Huovinen, T., Lindenburg, L., Minter, R. & Hollfelder, F. Multiplexed Affinity Characterization of Protein Binders Directly from a Crude Cell Lysate by Covalent Capture on Suspension Bead Arrays. Analytical Chemistry 93, 2166–2173 (2021).
Wood, S. M. et al. Cyanobacterial Akinete Distribution, Viability, and Cyanotoxin Records in Sediment Archives From the Northern Baltic Sea. Frontiers in Microbiology 12, 681881 (2021).
Peltomaa, R., Benito-Peña, E., Gorris, H. H. & Moreno-Bondi, M. C. Biosensing based on upconversion nanoparticles for food quality and safety applications. Analyst 146, 13-32 (2021).
Brandmeier, J. C. et al. Effect of Particle Size and Surface Chemistry of Photon-Upconversion Nanoparticles on Analog and Digital Immunoassays for Cardiac Troponin. Advanced Healthcare Materials 10, 2100506 (2021).
Gómez-Arribas, L. N. et al. Identification of High-Affinity Phage-Displayed VH Fragments by Use of a Quartz Crystal Microbalance With Dissipation Monitoring. Sensors and Actuators B: Chemical 340, 129954 (2021).
Luque-Uría, Á. et al. Recombinant Peptide Mimetic NanoLuc Tracer for Sensitive Immunodetection of Mycophenolic Acid. Analytical Chemistry 93, 10358–10364 (2021).

2020

Salminen, L. et al. A longitudinal analysis of CA125 glycoforms in the monitoring and follow up of high grade serous ovarian cancer. Journal of Gynecologic Oncology 156, 689–694 (2020).
Kaushik, N., Lamminmäki, U., Khanna, N. & Batra, G. Enhanced cell density cultivation and rapid expression-screening of recombinant Pichia pastoris clones in microscale. Scientific Reports. 10, 7458 (2020).
Salminen, L. et al. Exploratory analysis of CA125-MGL and -STn Glycoforms in the differential diagnostics of pelvic masses. The Journal of Applied Laboratory Medicine 5, 263–272 (2020).
Bayoumy, S. et al. Glycovariant-based lateral flow immunoassay to detect ovarian cancer–associated serum CA125. Communications Biology 3, 460 (2020).
Arponen, M., Brockmann, E-C., Kiviranta, R., Lamminmäki, U. & Ivaska, K.K. Recombinant Antibodies with Unique Specificities Allow for Sensitive and Specific Detection of Uncarboxylated Osteocalcin in Human Circulation. Calcified Tissue International 107, 529–542 (2020).
Kushnarova-Vakal, A., Äärelä, A., Huovinen, T., Virta, P. & Lamminmäki, U. Site-specific linking of an oligonucleotide to mono- and bivalent recombinant antibodies with SpyCatcher-SpyTag system for immuno-PCR. ACS Omega 5, 24927–24934 (2020).
Lindenburg, L. et al. Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins. Nucleic Acids Research 48, e63 (2020).
Peltomaa, R. et al. Bioluminescent detection of zearalenone using recombinant peptidomimetic Gaussia luciferase fusion protein. Microchimica Acta 187, 547 (2020).
Peltomaa, R. et al. Competitive upconversion-linked immunoassay using peptide mimetics for the detection of the mycotoxin zearalenone. Biosensors and Bioelectronics 170, 112683 (2020).